Abstract: The optimization of draw-off timing for a batch fermentation process is very important to increase the yields, productivity and to reduce energy consumption. A modelling approach of the heuristic fuzzy recognition and prediction to the draw-off timing is developed by using the fuzzy set theory and combining the statistics method with human experience. Meanwhile, according to the analysis of the historical data and the process of a practical industrial antibotic production, a model of the recognition and prediction to the draw-off timing of this process is established by means of the approach given in this paper. And, a real-time computer recognition system is designed. The result of several industrial tests shows that the effect of model recognition is satisfactory.